From last few decades latent genital tuberculosis has been a growing concern in developing countries like India and gaining more attention from healthcare providers especially in the context of in-vitro fertilisation. It may cause irreversible infertility and sometime affecting the entire reproductive system. In this report, we would discuss the nature of endometrial cytokines (both pro and anti) in case of latent genital tuberculosis, comparing with nonlatent genital tuberculosis and serum cytokines of the affected latent genital tuberculosis subjects. It has been observed that in most of the cases the growths of tuberculous bacilli in endometrium are arrested by natural defence of human body immune system mainly by T lymphocyte and macrophages. TH1 dependent cytokines activate macrophages resulting in protective immunity against local infection. But the presences of bacilli alter endometrial receptivity through pro-inflammatory cytokines by overburden mechanism, cause implantation failure. All pro-inflammatory cytokines have crossed margin value with statistically significant differences from subjects’serum cytokines level and non-latent genital tuberculosis cytokine level has been observed by studying 7 pro-inflammatory and 3 anti-inflammatory cytokines. In conclusion, the study confirms elevated level of endometrial pro-inflammatory cytokines in latent genital tuberculosis subjects indicating localised tuberculous infection. Latent genital tuberculosis invasion to the uterus in the form of infestation has been strongly recognised from this study.