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Characterisation of Amniotic Fluid Microbiome from Caesarean Section Mothers of Term Delivery

Soumita Dutta, Mahabub Alam, Reeta Rasaily, Reeta Bora, Utpala Devi, Analabha Basu, Suman K Paine, Dr Suman Kalyan Paine



Arguments of developmental biology like the sterility of womb during the foetus developmental stage are now countered by recent microbiome study in placenta and amniotic fluid. Methodological advancement like next generation sequencing technology identified a series of bacteria from placenta and amniotic fluid. However studies are limited for concrete conclusions on the sterility of wombs. Present report tried to explain two important question like (a) are amniotic fluid sterile which may be a supportive data about the sterility of womb, (b) if microbiota occurs then how the pattern of host-bacteria symbiosis exists in amniotic fluid.

Human microbial diversity argued as an ancient symbiosis, maintaining homeostasis, boosting host-immunity and growth but the initiation of microbial colonisation in the womb or after the birth seems unresolved and contradictory till date due to its sparse data.

Amniotic fluids were collected from 6 pregnant women who gave term birth attending from antenatal clinics of NE India, upon written informed consent in accordance with the Institutional-Ethics-Board. The present study reveals that amniotic fluid harbours 122.8+11.82 (SD) bacteria specific OTUs among the 6 samples that range from 111 to 141.  

The observation about more than 30% shared species between amniotic fluid and meconium samples which accounted for than 90% of total generated reads may signify microbial transmission of maternal-foetal during gestation may have potent impact on womb immune system and pre birth foetal microbial colonisation in the gut which may influence postnatal bacterial colonisation in the neonatal gut.


Microbiome, amniotic fluid, caesarean section, OTUs

Journal: Medical Glory Vol 6, Issue No 2, April-June, 2022


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